Final answer:
The most NADH is generated in the Tricarboxylic Acid (TCA) cycle, which contributes significantly to the production of ATP through oxidative phosphorylation.
Step-by-step explanation:
The pathway in which the most NADH is generated is the Tricarboxylic Acid (TCA) cycle, also known as the Krebs cycle or the citric acid cycle. In this cycle, pyruvate molecules from glycolysis are transformed into acetyl CoA and metabolized in the inner mitochondrial matrix. This process produces high-energy molecules, including NADH and FADH2. The NADH and FADH2 then donate electrons to the electron transport chain, which generates a substantial amount of additional ATP molecules through oxidative phosphorylation.