Final answer:
The base-pair sequence of a cDNA clone cannot be used to determine the sequence of the promoter, as cDNA is synthesized from mRNA, lacking regulatory sequences like promoters. Therefore correct option is D
Step-by-step explanation:
The information that cannot be determined from the base-pair sequence of a cDNA clone is the sequence of the promoter. cDNA is synthesized from an mRNA template and therefore represents the exon sequences of genes, which are the coding sequences that remain in mRNA after splicing. The promoter sequence, on the other hand, is a regulatory DNA sequence found upstream of the gene that controls the transcription process and is generally not part of the mRNA or cDNA sequence.
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