Question

you have a drug solution at a concentration of 64 um. if your goal is to make a 100 ul dilution at a final concentration of 16 um, how much volume of your initial stock do you need?

Answer 1

To prepare a 100 μl dilution at a concentration of 16 μm from a stock solution of 64 μm, you will need 25 μl of the stock solution.

Step-by-step explanation:

To determine how much volume of the initial 64 μm stock solution is needed to make a 100 μl dilution at a final concentration of 16 μm, you can use the dilution formula “C1V1 = C2V2”, where C1 is the concentration of the stock solution, V1 is the volume of the stock solution, C2 is the concentration of the final solution, and V2 is the volume of the final solution.

Plugging in the known values:

C1 = 64 μm

V1 = volume of stock solution needed (unknown)

C2 = 16 μm

V2 = 100 μl

Solving for V1, we get:

V1 = (C2 × V2) / C1

V1 = (16 μm × 100 μl) / 64 μm

V1 = 1600 / 64

V1 = 25 μl

Thus, you will need 25 μl of the 64 μm stock solution to make the 100 μl dilution at a final concentration of 16 μm.