Question

Which choice below lists the steps necessary for genetic cloning in the correct order?

O fragment the dna; insert dna into vector; insert vector into host; host replication of recombinant vector; isolate dna of interest
O isolate dna of interest; fragment the dna; insert dna into vector; insert vector into host; host replication of recombinant vector
O isolate dna of interest; fragment the dna; insert vector into host; insert dna into vector; host replication of recombinant vector
O isolate dna of interest; fragment the dna; host replication of recombinant vector; insert dna into vector; insert vector into host

Answer 1

The necessary steps for genetic cloning in the correct order involve isolating the DNA of interest, fragmenting it, inserting it into a cloning vector, and then introducing the vector into a host organism for replication.

Step-by-step explanation:

The correct order of steps for genetic cloning is: isolate DNA of interest; fragment the DNA; insert DNA into vector; insert vector into host; host replication of recombinant vector. First, the DNA of interest is isolated from the source, followed by fragmentation using restriction enzymes. This DNA is then inserted into a cloning vector, such as a plasmid. The recombinant vector is introduced into a suitable host organism, often a bacterium, which then replicates the vector along with the inserted DNA fragment, producing many copies of the desired gene.