30.4k views
1 vote
What is the original size of the linear DNA sample being digested by XhoI (Panel X), BamHI/XhoI (Panel B/X), and BamHI (Panel B)? Enter the number only _ _ kB

What is the order of the restriction sites in the linear DNA sample? Enter the single letter answer _ _
a. B/X/B
b. B/B/X
c. X/B/B/X
d. X/B/X
e. X/B/X/B

1 Answer

7 votes

Original size: 10 kB

Order of restriction sites: X/B/X/B. Hence the correct option is e.

The original size of the linear DNA sample undergoing digestion by XhoI (Panel X), BamHI/XhoI (Panel B/X), and BamHI (Panel B) is 10 kilobases (10 kB). The order of the restriction sites in the linear DNA sequence corresponds to the arrangement described as X/B/X/B. This indicates that the XhoI recognition site (X) is followed by the BamHI recognition site (B), and then another XhoI site, and finally a BamHI site.

This order represents the specific locations where the DNA is cleaved by the respective restriction enzymes in the given panels: XhoI, BamHI/XhoI, and BamHI. Each slash (/) denotes a cleavage site in the sequence. Hence the correct option is e.

User Wmz
by
8.5k points
Welcome to QAmmunity.org, where you can ask questions and receive answers from other members of our community.