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What is the original size of the linear DNA sample being digested by XhoI (Panel X), BamHI/XhoI (Panel B/X), and BamHI (Panel B)? Enter the number only _ _ kB

What is the order of the restriction sites in the linear DNA sample? Enter the single letter answer _ _
a. B/X/B
b. B/B/X
c. X/B/B/X
d. X/B/X
e. X/B/X/B

1 Answer

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Original size: 10 kB

Order of restriction sites: X/B/X/B. Hence the correct option is e.

The original size of the linear DNA sample undergoing digestion by XhoI (Panel X), BamHI/XhoI (Panel B/X), and BamHI (Panel B) is 10 kilobases (10 kB). The order of the restriction sites in the linear DNA sequence corresponds to the arrangement described as X/B/X/B. This indicates that the XhoI recognition site (X) is followed by the BamHI recognition site (B), and then another XhoI site, and finally a BamHI site.

This order represents the specific locations where the DNA is cleaved by the respective restriction enzymes in the given panels: XhoI, BamHI/XhoI, and BamHI. Each slash (/) denotes a cleavage site in the sequence. Hence the correct option is e.

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