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Which enzyme would cut this strand of DNA?

A) enzyme
Pst
B) enzyme
Hae III
C) enzyme
Hind III
D) enzyme
EcoRI
E) enzyme
Bam HI

1 Answer

4 votes

Final answer:

To identify which enzyme would cut a given DNA strand, the recognition sequence of each enzyme must be matched with the DNA sequence. BamHI and HaeIII are noted for producing sticky and blunt ends respectively, and EcoRI and HindIII produce sticky ends. Without the actual DNA sequence, the correct enzyme cannot be definitively identified.

Step-by-step explanation:

To determine which enzyme would cut a specific strand of DNA, we must first identify the recognition sequence for each of the enzymes listed as options for the student. According to the information provided:

  • BamHI recognizes a six-nucleotide palindrome and cuts the DNA to produce sticky ends.
  • HaeIII recognizes a four-nucleotide palindrome and cuts the DNA to produce blunt ends.
  • EcoRI recognition sequence is 5'GAATTC3' and it cuts within this sequence to produce sticky ends.
  • HindIII also produces sticky ends, but the student's question does not include its specific recognition sequence.

Without the actual DNA sequence to evaluate, we cannot definitively say which enzyme would cut the strand of DNA. However, we can say that each of these enzymes would be looking for its specific recognition sequence to bind and cut the DNA at specific points, either producing sticky or blunt ends, depending on the enzyme.

If the student provides the specific sequence of the DNA strand in question, then the correct enzyme can be identified based on which one's recognition sequence matches the DNA sequence. It's important that the recognition sequence is present in the DNA strand for the enzyme to cut it.

User Yiannr
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