Question

In figure 9.4, the bacteria transformed with the recombinant plasmid and plated on media containing ampicillin and x-gal will...

A) form blue, ampicillin-sensitive colonies
B) form white, ampicillin-resistant colonies
C) form blue, ampicillin-resistant colonies
D) not grow
E) form white, ampicillin-sensitive colonies

Answer 1

In figure 9.4, the bacteria transformed with the recombinant plasmid and plated on media containing ampicillin and x-gal will form white, ampicillin-resistant colonies.

Step-by-step explanation:

In figure 9.4, the bacteria transformed with the recombinant plasmid and plated on media containing ampicillin and x-gal will form white, ampicillin-resistant colonies. This is because the plasmids with an insert have a disrupted lacZ gene, which leads to the production of white colonies. On the other hand, plasmids lacking the foreign insert have an intact lacZ gene and are able to metabolize x-gal, resulting in blue colonies. Therefore, the correct answer is B) form white, ampicillin-resistant colonies.