Final answer:
The nitrogenous base determines differences in differing nucleotides.
Step-by-step explanation:
The component that determines differences in differing nucleotides is the nitrogenous base.
Nucleotides are the building blocks of DNA, and they consist of three components: a nitrogenous base, a pentose sugar, and a phosphate group.
The nitrogenous base can be either a purine, such as adenine (A) and guanine (G), or a pyrimidine, such as cytosine (C) and thymine (T).