Final answer:
In DNA replication, the reformation of the double helix is prevented by B) single-strand binding proteins.
Step-by-step explanation:
Single-strand binding proteins bind to the separated DNA strands to prevent them from rejoining and reforming the double helix. These proteins keep the DNA strands in an open, single-stranded state, allowing other enzymes to carry out the replication process.
Other enzymes involved in replication include DNA helicase, which separates the DNA strands, and DNA polymerases, which synthesize new DNA strands using the existing strands as templates. Therefore single-strand binding proteins prevent the reformation of the double helix in DNA replication. The correct answer is B) single-strand binding proteins.