Final answer:
The correct method for the Alpha-Amylase Defensins Experiment is Protein extraction, enzyme activity assay, and gel electrophoresis, where enzymes serve as the variable being assayed.Option B.
Step-by-step explanation:
The Alpha-Amylase Defensins Experiment likely aims to investigate the inhibition of enzyme activity or analyzing enzyme presence/functions in a biological system. Given the options provided, the most suitable method for such an experiment would be B: Protein extraction, enzyme activity assay, gel electrophoresis.
This sequence of steps involves isolating the protein (enzyme in this case), measuring its activity under various conditions to assess how defensins affect it, and using gel electrophoresis to separate and possibly quantify the proteins.
Enzymes in Western blotting function as markers to visualize the specific protein of interest. A chromogenic substrate is added, which, in the presence of the enzymatic marker attached to the secondary antibody, develops color, indicating the presence of the target protein.
In the context of analyzing enzyme activities, enzymes are not used as markers but rather as the studied variable whose activity is being assayed.
So Option B.