Question

How does pulse chase measure cell proliferation/turnover?

A)Assessing DNA replication rates
B)Quantifying protein synthesis and degradation rates
C)Counting the number of cells in a population
D)Analyzing cell cycle phase distribution

Answer 1

Pulse chase is a technique used to measure cell proliferation or turnover by following the movement of a labeled molecule through a cellular process. In the case of measuring cell proliferation/turnover, the pulse chase method can be used to assess DNA replication rates (option A).

Step-by-step explanation:

Pulse chase is a technique used to measure cell proliferation or turnover by following the movement of a labeled molecule through a cellular process. In the case of measuring cell proliferation/turnover, the pulse chase method can be used to assess DNA replication rates (option A). By introducing a labeled nucleotide precursor into the culture medium for a short period of time (the pulse), and then letting the cells continue to grow in normal medium (the chase), the labeled nucleotides are incorporated into newly synthesized DNA, allowing the measurement of DNA replication rates.