Final answer:
Stem cells are grown by culturing them on solid surfaces with the addition of specific growth factors, as proper adherence and growth factors are essential for their in vitro propagation.
Step-by-step explanation:
To grow stem cells according to Evans and Kaufman, the correct method is to culture on solid surfaces and the addition of specific growth factors.
This is because stem cells typically require a surface to adhere to for growth, such as plastic, glass, or other materials that also allow for microscopic observation. The culture medium provided needs to include the necessary nutrients, hormones, and stromal factors required for the growth and maintenance of these cells in vitro.
While primary cultures have a limited lifespan due to contact inhibition, where cells stop dividing when they come into contact with each other, manipulating the culture by transferring cells to new vessels with fresh medium can sustain growth. Continuous cell lines, on the other hand, may not show anchorage dependency and can grow indefinitely when subcultured properly with adequate medium changes.