Final answer:
The correct statement is B. HPLC can analyze samples over a wide polarity range and is able to analyze ionic samples. HPLC is a versatile technique, not limited by molecular weight, that uses a mobile phase and a stationary phase to separate and analyze compounds in a sample.
Step-by-step explanation:
The correct statement is B. HPLC can analyze samples over a wide polarity range and is able to analyze ionic samples.
HPLC, which stands for High Performance Liquid Chromatography, is a technique used to separate and analyze compounds in a sample. It utilizes a mobile phase and a stationary phase to achieve separation based on the affinity of the analytes for the stationary phase.
In the case of HPLC, the stationary phase is typically made of silica particles coated with hydrophobic chains. The analytes that are more hydrophobic will have stronger affinity to the stationary phase, resulting in slower movement through the column.
HPLC is a versatile technique that can be used to analyze samples with a wide range of polarities, including ionic samples. It is not limited by the molecular weight of the analytes, as stated in option A. And while there can be volatility issues with certain analytes, the statement in option C is incorrect, as the analyte does not necessarily have to be soluble in the mobile phase for HPLC analysis.