Question

Silica gel and Alumina produce a _____ ______ phase and both are generally used with nonpolar to moderately polar elution solvents as the mobile phase.

A) Polar-Stationary
B) Nonpolar-Stationary
C) Polar-Mobile
D) Nonpolar-Mobile

Answer 1

Silica gel and Alumina create a Nonpolar-Stationary phase used in reverse phase HPLC for separating hydrophobic compounds with nonpolar to moderately polar mobile phases.

Step-by-step explanation:

Silica gel and Alumina produce a Nonpolar-Stationary phase and are typically utilized with nonpolar to moderately polar elution solvents in the mobile phase. This setup is commonly used in reverse phase High-Performance Liquid Chromatography (HPLC), where the stationary phase is designed to attract and retain more hydrophobic compounds, causing them to move slowly through the column, thus facilitating separation based on hydrophobic interactions. Silica gel and alumina based columns provide a platform where the separation of molecules can be achieved, which is critical in the purification and analysis of compounds, including pharmaceuticals.