Final answer:
Adding a status change to a markup typically affects the color and thickness of lines or outlines. This visual differentiation helps to indicate a change in the review state or priority of the markup.
Step-by-step explanation:
When adding a status change to a markup in software, this typically affects visual elements such as color and thickness of lines or outlines, making the answer A) Color and Thickness. This might be because a status change often implies a change in the review state or priority of the marked-up item, and altering these properties helps to visually differentiate between different states.
Other options like opacity and fill, layer and font, as well as text and scale are generally not directly affected by status changes, although they can be modified independently in many markup tools. However, this will depend on the specific software or tool being used.