Question

Expressed sequence tags (ESTs) are synthesized and spotted onto glass slides. cDNA made from RNA of two cell sources, labeled with different fluorescent dyes. cDNAs pooled and hybridized to ESTs on chip, excitation and measurement of intensities of fluorescence (therefore degree of hybridization of each sample's cDNA to each EST).

A) DNA Microarray
B) Southern Blot
C) Northern Blot
D) Western Blot

Answer 1

The described technique, which involves synthesizing ESTs, labeling cDNA from two cell types, and hybridizing them to a chip for fluorescence measurement, is known as a DNA microarray.

Step-by-step explanation:

The technique described involves the synthesis of expressed sequence tags (ESTs), which are then spotted onto glass slides to create a microarray. cDNA is synthesized from RNA derived from two different cell sources and labeled with distinct fluorescent dyes. These labeled cDNAs are combined, or pooled, and hybridized to the ESTs on the chip. After hybridization, the fluorescence intensities are excited and measured, reflecting the degree of hybridization of each sample's cDNA to the individual ESTs. This procedure has the hallmarks of a DNA microarray assay, which allows for the simultaneous analysis of the expression of many genes.