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Acetoacetate decarboxylase catalyzes the decarboxylation of acetoacetate via the formation of Schiff base between the ketone of acetoacetate and its Lys 115. Which of the following is most likely correct?

a) The measured pKa of Lys 115 is 10.54
b) Lys 115 is a potent electrophile
c) The pKa of Lys 115 is independent of its microenvironment
d) The perturbed pKa of Lys 115 is a result of interaction with Glu 76

User Serializer
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Final answer:

The perturbed pKa of Lys 115 in acetoacetate decarboxylase is most likely due to the interaction with Glu 76, which would suggest a nucleophilic role for Lys 115 in the Schiff base formation involved in the enzyme's catalytic mechanism.

Step-by-step explanation:

Acetoacetate decarboxylase catalyzes the decarboxylation of acetoacetate through the formation of a Schiff base between the ketone of acetoacetate and Lys 115. The most likely correct answer regarding Lys 115 in this context is that the perturbed pKa of Lys 115 is a result of the interaction with Glu 76. A pKa of 10.54 would be unusually high for a lysine in a typical protein environment, indicating that the pKa is most likely perturbed. A Schiff base formation would suggest that Lys 115 acts as a nucleophile rather than an electrophile. The pKa of amino acid residues within enzymes can be profoundly influenced by their microenvironment, thus it is unlikely to be independent.

User Gal Dreiman
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