Question

For this, you need reverse transcriptase, dNTPs, dT15 primer, and mRNA template.

A) PCR
B) Northern Blot
C) cDNA Synthesis
D) Southern Blot

Answer 1

Reverse transcriptase PCR (RT-PCR) is the process used to amplify DNA fragments from an RNA template by reverse transcription and subsequent PCR amplification.

Step-by-step explanation:

Reverse transcriptase PCR (RT-PCR) is the process used to amplify DNA fragments from an RNA template. The first step is reverse transcription, where the RNA template is converted into complementary DNA (cDNA) using the enzyme reverse transcriptase.

In this process, reverse transcriptase, dNTPs, dT15 primer, and mRNA template are required. The reverse transcriptase enzyme acts as a catalyst to synthesize the cDNA using the mRNA template. Once the cDNA is created, regular PCR is used to amplify it. PCR involves the use of primers and DNA polymerase to make multiple copies of the cDNA sequence.

The process described in the question involves the use of reverse transcriptase, dNTPs, dT15 primer, and an mRNA template, which are components used for cDNA synthesis. In this process, reverse transcriptase synthesizes a complementary DNA (cDNA) strand from an RNA template, in a reaction that is reverse to the usual transcription of DNA to RNA. This step is followed by PCR amplification of the cDNA if needed. The correct answer to the question is C) cDNA Synthesis.