Question

Restriction enzyme recognizes a specific DNA sequence and cuts it to produce two sticky ends. Two different DNA molecules with matching sticky ends can bind each other.

A) Ligase
B) Polymerase Chain Reaction (PCR)
C) Southern Blot
D) Restriction Digest

Answer 1

DNA ligase is the enzyme that glues together DNA fragments with matching sticky ends, created by restriction enzymes, to form recombinant DNA.

Step-by-step explanation:

The specific DNA sequence recognized and cut by a restriction enzyme to produce two sticky ends facilitates the binding of two different DNA molecules with matching ends. The enzyme responsible for gluing these annealed DNA fragments together is known as DNA ligase. This process is integral to the construction of recombinant DNA, where DNA fragments are combined to form new genetic sequences. Restriction enzymes are used extensively to cut DNA fragments at specific, typically palindromic sequences, creating either sticky ends or blunt ends, depending on the enzyme. After digestion with restriction enzymes, the DNA fragments can then be annealed and subsequently joined by DNA ligase to form a continuous DNA strand, completing the recombination process.