Final answer:
RNase is the enzyme used to partially digest the RNA template in the process of DNA replication, particularly for removing RNA primers from Okazaki fragments.
Step-by-step explanation:
The enzyme responsible for partially digesting the RNA template is RNase. RNase is a nuclease that catalyzes the degradation of RNA into smaller components. It is often used in DNA replication to remove RNA primers from Okazaki fragments on the lagging strand, and the remaining gaps are then filled by DNA polymerase and sealed by DNA ligase. DNA polymerase is also integral in this process as it catalyzes the synthesis of DNA using an existing strand of DNA as a template. DNA polymerase I in particular has a 5'→3' exonuclease activity which can remove RNA primers at the ends of Okazaki fragments.