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Used to measure mRNA half-lives, which gives stability. Cells are treated with a transcriptional inhibitor for different lengths of time. Since transcription is prevented, the amount of mRNA at any time after is proportional to the rate of decay. Purify RNA then quantify using northern blot, quantitative PCR, etc. Then plot on a curve and determine half-life.

A. mRNA stability assay
B. mRNA decay analysis
C. mRNA half-life determination
D. mRNA pulse-chase experiment

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Final answer:

The process of measuring mRNA half-life involves using transcriptional inhibitors and quantifying the rate of decay using methods like quantitative PCR, providing insights into mRNA's stability and consequently, protein synthesis.

Step-by-step explanation:

Cells utilize various methods to measure and control the RNA stability, which in turn affects protein synthesis within the cell. When examining the mRNA half-life, researchers typically employ a method where cells are treated with a transcriptional inhibitor to halt mRNA synthesis. Thereafter, the remaining mRNA levels are measured over different periods, reflecting their rate of decay. This decay rate, or the mRNA's halving of its initial quantity, provides insight into the molecule's stability. Common techniques such as northern blot and quantitative PCR are used to quantify RNA after purification, and then data is plotted to determine the half-life of the mRNA.

To provide protective measures against degradation, mRNA is capped at the 5' end with a methylated GTP and at the 3' end with a poly-A tail. Also, the longevity of mRNA in the cytoplasm is controlled by factors including RNA-binding proteins and microRNAs. This RNA stability influences the duration the mRNA remains available for protein translation. Furthermore, the steady-state level of proteins in a cell depends on the balance between synthesis and turnover, where the half-life plays a crucial role in regulating this balance.

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