Question

Scientists combined the function of tracr and crRNA to make a guide RNA (gRNA) that directly associates with Cas9 to cut DNA, then NHEJ repairs the double-strand break but in the process of repair, we get deletions/insertions--> stop codons--> KO gene.

A) CRISPR-Cas9
B) RNAi
C) Ligase
D) Restriction Enzyme

Answer 1

The subject of the question is CRISPR-Cas9, which is a gene editing technique that combines the function of tracr and crRNA to create a guide RNA (gRNA) that associates with Cas9 to cut DNA. This process can lead to deletions or insertions in the DNA, resulting in the knockout of a gene.

Step-by-step explanation:

The subject of the question is CRISPR-Cas9.

CRISPR-Cas9 is a gene editing technique that combines the function of tracr and crRNA to create a guide RNA (gRNA) which associates with Cas9 to cut DNA. The double-strand break caused by Cas9 is repaired by Non-Homologous End Joining (NHEJ), which can sometimes lead to deletions or insertions that result in the creation of stop codons in the gene, resulting in a knockout (KO) of the gene.

Keywords: CRISPR-Cas9, tracr, crRNA, guide RNA, gRNA, Cas9, DNA, NHEJ, deletions, insertions, stop codons, KO gene