Final answer:
Class II aminoacyl-tRNA synthetases attach amino acids to the 3'-OH of the tRNA's terminal adenylate which is critical for the tRNA charging process.
Step-by-step explanation:
Class II aminoacyl-tRNA synthetases add the amino acid to the 3'-OH of the terminal adenylate. This addition of an amino acid to the end of a tRNA molecule is a crucial part of the process called tRNA "charging".
During this process, an enzyme named aminoacyl tRNA synthetase catalyzes the attachment of a specific amino acid to the 3' end of tRNA, facilitating the accurate translation of mRNA into proteins.