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How can you analyze fractions to measure the total amount of protein?

A) Gel electrophoresis
B) Mass spectrometry
C) Chromatography
D) Bradford assay

User Flodel
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1 Answer

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Final answer:

The correct method to analyze fractions and measure the total amount of protein is the Bradford assay, which quantifies protein concentration through a colorimetric reaction.

Step-by-step explanation:

To analyze fractions and measure the total amount of protein in a sample, the Bradford assay is a suitable method. Unlike techniques such as gel electrophoresis and mass spectrometry which separate and characterize proteins, the Bradford assay quantifies the protein concentration. This assay relies on a color change produced by the binding of a dye to proteins, with the color intensity corresponding to protein concentration. By comparing the absorbance measurement to a standard curve, the total amount of protein can be quantified.

User Marc Wittke
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