Final answer:
Integral membrane proteins need to be gently removed using detergents to avoid denaturation and preserve their functionality. The correct option is B.
Step-by-step explanation:
To remove integral membrane proteins, which are integrated into the membrane structure and interact extensively with the hydrocarbon chains of membrane lipids, gentle conditions are required, such as the use of detergents.
Unlike peripheral proteins that can be easily isolated, integral membrane proteins require the disruption of the membrane to be extracted, because their hydrophobic alpha-helical domains are established within the membrane's hydrophobic core.
Denaturation methods such as high temperatures, extreme pH levels, and organic solvents can also denature proteins by affecting their three-dimensional structure. However, to preserve the function and structure of integral membrane proteins during their extraction, using mild and non-disruptive agents like detergents is preferred.