Final answer:
The absence of positively stained cells in a Grimelius-stained section of ileum is most likely due to c. the use of an old developer. This conclusion is aligned with the principles of histological staining techniques.
Step-by-step explanation:
The question involves the interpretation of histological staining outcomes using the Grimelius technique, which is a silver staining method used for detecting certain types of cells, such as neuroendocrine cells.
If a section of ileum stained with the Grimelius technique does not demonstrate any positively stained cells, this is most likely the result of the use of an old developer.
This answer is based on the provided methods of staining, such as the Ziehl-Neelsen and Gram staining techniques.
These methods involve a process of applying primary stains, decolorizing agents, and sometimes a secondary counterstain, like safranin or methylene blue.
Problems with the staining process, like using an old developer, can lead to an absence of positively stained cells.
In histology, staining is crucial for visualizing structures within tissues, and the proper preparation and use of reagents are critical for achieving accurate results.
Factors such as the concentration of the staining agents, the timing of each step, and the freshness of the reagents all can influence the outcome of the staining procedure.