Question

Control sections stained with Prussian Blue show diffuse blue staining, and the solution is also blue. One possible explanation is that the:

a. slides were incubated for 1 hour at room temperature
b. control chosen contains a soluble form of iron
c. staining jar was contaminated
d. section was too thick

Answer 1

The most likely explanation for diffuse blue staining in control sections using Prussian Blue is that the control contains a soluble form of iron.

Step-by-step explanation:

The Prussian Blue staining technique is used for identifying iron deposits in tissue sections. If control sections stained with Prussian Blue show diffuse blue staining and the solution itself is also blue, one possible explanation is that the control chosen contains a soluble form of iron. This may result from selecting a sample with iron that easily dissolves in the staining solution, leading to a diffuse blue color throughout the section rather than localized staining where iron deposits are present.