Final answer:
EcoRI recognizes the sequence 5'-G|AATTC-3' ("|" indicates the point of cleavage). Treatment of the following oligonucleotide with EcoRI would produce two oligonucleotides with sizes of c) 22, double-stranded nucleotides containing cohesive ends. 5'-CAAGTCGATACAGAATTCGTACCTAG-3'
Step-by-step explanation:
Treatment of the given oligonucleotide with EcoRI, which recognizes the sequencec) 5'-G|AATTC-3', would produce two oligonucleotides with sizes of 22 double-stranded nucleotides containing cohesive ends. The cleavage point is at the EcoRI recognition sequence, resulting in two fragments with complementary cohesive ends.
EcoRI cleaves the given oligonucleotide at the G|AATTC site, creating two fragments. The original oligonucleotide is 26 nucleotides long, and cleavage occurs between the G and AATTC.
The sizes of the resulting fragments are 5'-CAAGTCGATACAG-3' and 5'-AATTCGTACCTAG-3', both 22 nucleotides long. The cohesive ends are complementary, as one strand has an overhang of AATTC, and the other has the complementary sequence, forming cohesive or sticky ends.
Understanding restriction enzyme recognition sequences and their cleavage patterns is fundamental in molecular biology. EcoRI is a commonly used restriction enzyme with a recognition site that generates cohesive ends upon cleavage. These cohesive ends facilitate the ligation of DNA fragments in genetic engineering and recombinant DNA technology.