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If histidine (pKa=6.04) is being used by an enzyme that catalyzes the conversion of a ketone to an enol by base catalysis; which of the following would be most advantageous to have in the vicinity of the active site histidine in a pH=5 environment?

a) Aspartic acid
b) Glutamic acid
c) Serine
d) Lysine

User Pbibergal
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1 Answer

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Final answer:

In a pH 5 environment, lysine would be the most advantageous to have in the vicinity of the active site histidine for base catalysis, as it is basic and helps histidine act as a base in the catalytic process.

Step-by-step explanation:

If histidine (pKa=6.04) is being used by an enzyme that catalyzes the conversion of a ketone to an enol by base catalysis, and the environment is at a pH of 5, the most advantageous amino acid to have in the vicinity of the active site histidine would be lysine (d).

Lysine is a basic amino acid with a higher pKa value for its side chain, which helps histidine accept a proton more readily, facilitating its role as a base in the catalytic process. In an environment with a pH lower than the pKa of histidine, its ability to act as a base would be compromised because it would be more protonated and less able to remove a proton from the substrate. Lysine, being basic, increases the local pH, thus promoting the deprotonation of histidine needed for base catalysis.

Amino acids like aspartic acid, glutamic acid, and serine, because of their different side chain chemistries and pKa values, are less likely to enhance the base catalytic activity of histidine under these conditions.

User Praveen VR
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