Question

What are the products from treatment of the following peptide with trypsin? Leu-Val-Ala-Arg-Phe-Val-Lys-Asn-Leu-Gly-Arg-Cys-Asp

a) Leu-Val-Ala
b) Arg-Phe
c) Val-Lys-Asn-Leu
d) Gly-Arg-Cys-Asp

Answer 1

The products from the treatment of the given peptide with trypsin are Leu-Val-Ala-Arg, Phe-Val-Lys, Asn-Leu-Gly-Arg, and Cys-Asp, as trypsin cleaves after arginine and lysine residues.

Step-by-step explanation:

The question involves determining the products of a peptide treated with trypsin. Trypsin is a proteolytic enzyme that specifically cleaves peptide bonds at the carboxyl side of lysine (Lys) and arginine (Arg) residues, except when either is followed by proline. Given the peptide sequence Leu-Val-Ala-Arg-Phe-Val-Lys-Asn-Leu-Gly-Arg-Cys-Asp, trypsin will cut after Arg and Lys, which gives us the following peptides: Leu-Val-Ala-Arg | Phe-Val-Lys | Asn-Leu-Gly-Arg | Cys-Asp. Therefore, the correct products are Leu-Val-Ala-Arg, Phe-Val-Lys, Asn-Leu-Gly-Arg, and Cys-Asp.