Final answer:
The statement regarding the use of DNA polymerase in DNA amplification, DNA ligase in cDNA and DNA vector joining, and reverse transcriptase in cDNA synthesis is true. These enzymes are essential for various biotechnological applications, including PCR amplification, gene cloning, and studying gene expression.
Step-by-step explanation:
The statement that DNA polymerase is used in DNA amplification, DNA ligase is used in cDNA and DNA vector joining, and reverse transcriptase is used in the creation of cDNA is A) True.
DNA polymerase is an enzyme that is instrumental in the process of DNA replication, adding nucleotides to a growing DNA strand. The polymerase chain reaction (PCR) utilizes a specific type of DNA polymerase called Taq polymerase for DNA amplification. PCR is a method used to make numerous copies of a specific segment of DNA rapidly, and Taq polymerase is essential because it can withstand the high temperatures required during the PCR cycles.
DNA ligase is another key enzyme that plays a vital role in molecular biology. It seals nicks and joins fragments of DNA together, such as when combining cDNA with a DNA vector to create recombinant DNA molecules. This is a crucial step in biotechnological applications like gene cloning and genetic engineering.
Reverse transcriptase is used in reverse transcriptase PCR (RT-PCR), which involves the synthesis of cDNA from an RNA template before the amplification step of PCR. This allows DNA fragments to be amplified from an RNA molecule, which is a critical technique for studying gene expression.