Question

List the steps of dilution plating.

Answer 1

The steps of dilution plating involve preparing a series of dilutions, plating samples on a growth medium, incubating the plates, and counting the colonies.

Step-by-step explanation:

The steps of dilution plating are as follows:

1. Prepare a series of dilutions by adding a fixed volume of the original culture to a tube containing sterile dilution solution. This is done multiple times to create a range of dilutions.
2. Take a small sample from each dilution tube and plate it on a solid growth medium using either the pour plate or spread plate method.
3. Incubate the plates until colonies appear.
4. Count the number of colonies on each plate and average the counts to estimate the number of cells in the original culture.