Question

How to calculate cells in a counting chamber?

Answer 1

To calculate cells in a counting chamber, follow the steps of placing a known volume of the culture suspension, counting the cells in small squares, and calculating the average number of cells.

Step-by-step explanation:

The cells in a counting chamber, specifically a Petroff-Hausser chamber, can be calculated by following these steps:

1. Place a known volume of the culture suspension onto the chamber under a coverslip at a specific height from the surface of the grid.
2. Count the cells in several small squares of known area within the chamber.
3. Calculate the average number of cells counted per square.
4. Use the average number of cells per square to estimate the concentration of cells in the original sample.
5. Correct the concentration for dilution if necessary.