Final answer:
To calculate cells in a counting chamber, follow the steps of placing a known volume of the culture suspension, counting the cells in small squares, and calculating the average number of cells.
Step-by-step explanation:
The cells in a counting chamber, specifically a Petroff-Hausser chamber, can be calculated by following these steps:
- Place a known volume of the culture suspension onto the chamber under a coverslip at a specific height from the surface of the grid.
- Count the cells in several small squares of known area within the chamber.
- Calculate the average number of cells counted per square.
- Use the average number of cells per square to estimate the concentration of cells in the original sample.
- Correct the concentration for dilution if necessary.