Final answer:
The limitation of relying solely on 16S similarity to determine species is addressed by DNA-DNA hybridization. Protein sequencing and cell morphology analysis are also techniques that can provide additional information in species determination. option A is answer
Step-by-step explanation:
The limitation of relying solely on 16S similarity to determine species is addressed by DNA-DNA hybridization. DNA-DNA hybridization is a technique that measures the degree of similarity between the DNA of two organisms. By comparing the DNA of different organisms, scientists can determine their relatedness and classify them into different species.
Another technique that can provide additional information in species determination is protein sequencing. Proteins are made up of amino acids, and the sequence of these amino acids varies between different species. By comparing the protein sequences of different organisms, scientists can determine their relatedness and classify them into different species. option A is answer