Final answer:
The exact time required for generating HHPred and NCBI matches using PECAAN is not provided, but it is influenced by server load, query complexity, and sequence length. Mass spectrometry and peptide mapping are key for protein identification, which is central to tools like PECAAN.
Step-by-step explanation:
The duration for generating HHPred and NCBI matches to gene products using PECAAN is not specified in the provided references. However, generally, the time can vary depending on several factors including server load, query complexity, and sequence length. Tools like MASCOT, which is mentioned for protein identification using mass spectrometry data, perform database searches utilizing certain parameters such as those outlined for plant proteins: Database (NCBInr), taxonomy (green plants), enzyme (trypsin), modifications, peptide mass tolerance, fragment mass tolerance, and statistical significance. These parameters are key for accurate peptide mapping and protein identification which are essential for downstream processes like functional analysis performed via tools like GO web-tool or databases like P3DB for phosphoprotein analysis.