Question

Limitations to shotgun sequencing method: Cloning bias?

a) Bias in replicating DNA
b) Bias in selecting specific sequences
c) Bias in amplifying genes
d) Bias in incorporating nucleotides

Answer 1

The limitations to shotgun sequencing method include a bias in selecting specific sequences, potentially resulting in an incomplete or skewed representation of the original DNA sequence. option b is answer

Step-by-step explanation:

The limitations to shotgun sequencing method include a bias in selecting specific sequences. When DNA fragments are cut randomly into smaller pieces during shotgun sequencing, there is a possibility of biased selection, meaning some sequences may be more likely to be selected than others. This can result in an incomplete or skewed representation of the original DNA sequence.

For example, imagine a DNA sequence that contains repeating segments. If the shotgun sequencing method is biased and tends to select shorter fragments more often, those repeating segments may be underrepresented or entirely missed in the final assembled sequence.

It is important for scientists to be aware of this limitation and account for it when interpreting and analyzing shotgun sequencing data. option b is answer