Final answer:
Second Generation Sequencing involves DNA sequencing and the general structure used is Linear DNA (b), which is part of a double helix formed by two single DNA strands hydrogen bonded together.
Step-by-step explanation:
Second Generation Sequencing, also known as high-throughput sequencing, involves DNA as the genetic material that undergoes sequencing. The correct answer is b) Linear DNA. The general structure of DNA is a double helix, as noted in figures across various biology textbooks, such as Figure 20.18, which shows DNA as a double helix consisting of two single DNA strands hydrogen bonded together at each nitrogenous base. During the sequencing process, chromosomes are broken down into smaller pieces, sometimes resulting in linear DNA fragments that are sequenced in parallel to achieve rapid data output.
The structure of DNA is commonly described in textbooks with illustrations like Figure 21.18, which describes the DNA molecule with a double helix design, consisting of a backbone of sugars and phosphate groups and nitrogenous bases paired through hydrogen bonds. This contrasts with RNA, which is typically single-stranded and capable of folding upon itself as shown in Figure 10.21.