Question

Explain in detail the background, theoretical and practical aspects of the extraction procedure as well as the instrmental assay used in the analysis of Cocaine and Amphetamines in Oral Fluid by LC-MS/MS. (Start by define cocaine and amphetamines, their peak concentration, window of detection in oral fluid, blood and urine. Define oral fluid and how oral fluid sample is collected for analysis, define LC-MS/MS and how it is used/employed to analyze cocaine and amphetamine in oral fluid). Please explain with these points i stipulated in bracket, dont just post an article of the experiment i need a clear detailed information. This is much appreciated.

Answer 1

The extraction procedure and instrumental assay used to analyze cocaine and amphetamines in oral fluid is LC-MS/MS. This technique separates the compounds in a sample and uses mass spectrometry to detect and quantify them. Cocaine and amphetamines have specific peak concentrations and detection windows in oral fluid.

Step-by-step explanation:

To analyze cocaine and amphetamines in oral fluid, the extraction procedure and instrumental assay used is LC-MS/MS. Cocaine is a powerful stimulant and amphetamines are a group of synthetic drugs that also act as stimulants. The peak concentration of cocaine in oral fluid is generally around 10-100 ng/mL, while for amphetamines it is around 100-1000 ng/mL. The detection window for both cocaine and amphetamines in oral fluid is typically within 2-5 days. Oral fluid samples are collected by using a collection device that has an absorbent pad placed in the mouth, and the pad absorbs the saliva. LC-MS/MS, which stands for liquid chromatography-tandem mass spectrometry, is a technique used to separate and analyze compounds in a sample. In this case, it is used to analyze cocaine and amphetamines in oral fluid. The technique involves passing the sample through a liquid chromatography column to separate the compounds, and then using mass spectrometry to detect and quantify them.