Final answer:
The extraction procedure and instrumental assay used to analyze cocaine and amphetamines in oral fluid is LC-MS/MS. This technique separates the compounds in a sample and uses mass spectrometry to detect and quantify them. Cocaine and amphetamines have specific peak concentrations and detection windows in oral fluid.
Step-by-step explanation:
To analyze cocaine and amphetamines in oral fluid, the extraction procedure and instrumental assay used is LC-MS/MS. Cocaine is a powerful stimulant and amphetamines are a group of synthetic drugs that also act as stimulants. The peak concentration of cocaine in oral fluid is generally around 10-100 ng/mL, while for amphetamines it is around 100-1000 ng/mL. The detection window for both cocaine and amphetamines in oral fluid is typically within 2-5 days. Oral fluid samples are collected by using a collection device that has an absorbent pad placed in the mouth, and the pad absorbs the saliva. LC-MS/MS, which stands for liquid chromatography-tandem mass spectrometry, is a technique used to separate and analyze compounds in a sample. In this case, it is used to analyze cocaine and amphetamines in oral fluid. The technique involves passing the sample through a liquid chromatography column to separate the compounds, and then using mass spectrometry to detect and quantify them.