Final answer:
After dialysis, the molecular weight of proteins decreases, while Amicon centrifugal concentration increases the concentration of the target protein T7RNAP.
Step-by-step explanation:
After dialysis, you can expect to see a decrease in the molecular weight of the proteins. Dialysis removes small molecules such as salts and urea, leaving behind the larger protein molecules. This can be observed through techniques like gel electrophoresis, which separates proteins based on their molecular weight.
After Amicon centrifugal concentration, you would expect to see an increase in the concentration of your target protein, T7RNAP. The centrifugal concentration process removes excess solvent, allowing the protein of interest to be concentrated. This can be confirmed through techniques such as gel electrophoresis or spectrophotometry.