Final answer:
If the gene encoding the enzyme rubisco is mutated and non-functional, it would affect the process of carbon fixation in photosynthesis.
Step-by-step explanation:
The gene encoding the enzyme rubisco plays a crucial role in the process of carbon fixation, which is part of the light-independent reactions in photosynthesis. The enzyme catalyzes the addition of carbon dioxide (CO₂) to a molecule called ribulose bisphosphate (RuBP), resulting in the formation of 3-phosphoglycerate (3-PGA). This step is essential for the production of carbohydrate molecules.
If the gene encoding rubisco is mutated and non-functional, the ability to fix carbon dioxide would be affected. This would disrupt the Calvin cycle and hinder the formation of glyceraldehyde 3-phosphate (G3P), which is the precursor to carbohydrate synthesis.