Final answer:
The statement that Group D Strep is bile esculin negative is false, as Group D Strep (Enterococcus spp.) is known to be bile esculin positive and would hydrolyze esculin in the presence of bile, changing the medium's color.
Step-by-step explanation:
Group D Strep is typically identified as Enterococcus spp., which are known for being bile esculin positive. Given that Bile Esculin Agar is a medium specifically used to isolate and identify enterococci by their ability to hydrolyze esculin in the presence of bile, Group D Strep would turn the medium brownish-black. Therefore, the statement that Group D Strep is bile esculin negative is False. Other species, like Streptococcus pyogenes which belongs to Group A, would not grow in such a selective medium since they are not bile tolerant.