Final answer:
The statement is false. A light blue stopper tube should not be collected first when using a butterfly. It is not specific to the use of a butterfly.
Step-by-step explanation:
The statement is false. When using a butterfly, a light blue stopper tube should not be collected first.
In fact, a light blue stopper tube is used to collect the uppermost layer of a liquid after a separation process, such as decantation or centrifugation.
It is not specific to the use of a butterfly.