Final answer:
A copy of the DOT final rule must be on file with the Department of Transportation (DOT) and it includes regulations and guidelines related to animal transportation.
Step-by-step explanation:
A copy of the DOT final rule must be on file with the Department of Transportation (DOT). This means that the DOT has a record of the final rule and it is available for reference by the DOT and other relevant parties. The DOT final rule is a comprehensive set of regulations and guidelines that govern various aspects of transportation, including the transportation of animals. It includes forms that attest to the health, behavior, and training of the animals being transported.