Question

Which statement is NOT a reason we added arabinose to the transformation growth media?

A) Arabinose sugar binds to the AraC repressor protein, removing it from the PBAD promoter.
B) Arabinose was needed to induce expression of the GFP gene by allowing transcription from the PBAD promoter.
C) Arabinose is a sugar, so it is added to the LB media to promote the growth of the E. coli.
D) None of the above

Answer 1

Arabinose is added to transformation growth media to induce GFP gene expression, not to promote E. coli growth, which makes statement C incorrect.

Step-by-step explanation:

The statement that is NOT a reason for adding arabinose to the transformation growth media is "C) Arabinose is a sugar, so it is added to the LB media to promote the growth of the E. coli." The reason arabinose is added to the growth media during a transformation experiment is to induce expression of the GFP gene. This is achieved by arabinose binding to the AraC repressor protein, which removes it from the PBAD promoter as stated in option A), and thereby allowing transcription from the PBAD promoter as mentioned in option B).