Question

Prior to analysis of protein structure, a pure sample of that protein must be enriched. For isolation of cytochrome c following mitochondrial isolation and subsequent lysis, the_________fraction must be further purified.

Answer 1

After mitochondrial fractionation, the mitochondrial fraction must be purified further to isolate cytochrome c. This process is simplified as cytochrome c easily dissociates from the mitochondrial membrane.

Step-by-step explanation:

For isolation of cytochrome c following mitochondrial isolation and subsequent lysis, the mitochondrial fraction must be further purified. Cytochrome c, a peripheral membrane protein, easily dissociates from the inner mitochondrial membrane, simplifying its purification. After mitochondrial fractionation, additional purification steps such as column chromatography, differential centrifugation, or specific isolation techniques like immunoaffinity purification can be performed to enrich the sample of cytochrome c.