Question

The apparatus shown measures the oxygen consumed by a small animal as oxygen is consumed. The water droplet moves closer to the flask, because carbon dioxide is absorbed by the potassium hydroxide solution on the filter paper. It does not affect the results. Your performing a lab biology class to determine quantitatively if temperature affects the rate of oxygen consumption in a small animal you’ll be testing the oxygen consumption of the animal at 0° 15° degrees and 30° degrees Using the apparatus or similar one right procedure to test your prediction procedures should be detailed specific and written in number point form

Answer 1

To measure the oxygen consumption of an animal at different temperatures, use a respirometer with potassium hydroxide to absorb CO2. Maintain temperatures at 0°C, 15°C, and 30°C, monitor the water droplet movement, and record oxygen consumption to determine the respiration rate.

Step-by-step explanation:

To accurately measure the rate of oxygen consumption of a small animal at different temperatures using a respirometer, follow this detailed procedure:

1. Calibrate the respirometer by placing it in water at known temperatures (0°C, 15°C, and 30°C) and record initial readings.
2. Insert the small animal into the respirometer and ensure it is sealed tightly to prevent air from escaping.
3. Add potassium hydroxide (KOH) solution to the apparatus to absorb carbon dioxide produced by the animal, allowing for a more accurate measurement of oxygen consumption.
4. Maintain each temperature condition using an ice bath for 0°C, room temperature for 15°C, and a warm water bath for 30°C.
5. Observe the movement of the water droplet in the pipette as oxygen is consumed by the animal. The droplet will move closer to the flask indicating oxygen uptake.
6. Take readings of oxygen consumption at regular intervals (e.g., every minute) for a set duration (e.g., 15-20 minutes), ensuring that temperature is kept constant throughout the experiment.
7. Calculate the rate of oxygen consumption by noting the volume displacement over time and adjusting for the mass of the animal.
8. Repeat the procedure for each temperature point (0°C, 15°C, and 30°C) to gather comparative data.
9. Analyze the data to determine the effect of temperature on the rate of respiration of the animal.

This systematic approach helps in making accurate conclusions regarding the impact of temperature on metabolic activities.