Final answer:
The ligation of alien DNA at the Hind III restriction site can be successful if sticky ends are compatible and properly ligated by DNA ligase. Agarose gel electrophoresis can be used to assess the success of the cloning process.
Step-by-step explanation:
The experiment can be successful if the alien DNA is ligated at the Hind III restriction site, provided that the alien DNA fragment and the plasmid have compatible ends created by the Hind III enzyme. Restriction enzymes like Hind III cut the DNA at specific sequences, creating sticky ends, which are single-stranded overhangs.
These sticky ends can pair with complementary sequences on another piece of DNA cut by the same enzyme. After pairing, DNA ligase is used to covalently link the sugar-phosphate backbones of the DNA to 'glue' the fragmens together, forming a stable molecule.
If the alien DNA was cut by Hind III and has complementary sticky ends to those on the plasmid, and no mutations or hindrances occur at the ligation site, ligation can successfully occur following proper alignment and ligation reactions. The success of the ligation can be assessed via agarose gel electrophoresis, which allows verification of the size and number of DNA fragments to infer successful incorporation.