Question

Suppose that a typical cell is approximately 1/3 the diameter of the field of vision using high power and the diameter of the field of vision for high power is 600 micrometers. What is the size of this cell? (one millimeter = 1,000 micrometers)

How does increasing the magnification affect the field of vision?
Explain how total magnification is calculated.
Why is it important to avoid creating air bubbles from the slide when preparing a wet mount?

Answer 1

The size of the cell is approximately 200 micrometers. Increasing magnification decreases the field of vision. Creating air bubbles from the slide can interfere with image clarity.

Step-by-step explanation:

In this case, the size of the cell can be calculated by multiplying the diameter of the field of vision (600 micrometers) by the fraction that represents the size of the cell compared to the field of vision (1/3). So, the size of the cell is 600 micrometers * (1/3) = 200 micrometers.

Increasing the magnification of a microscope decreases the field of vision, meaning you can see less of the specimen at one time. Total magnification is calculated by multiplying the magnification of the objective lens by the magnification of the eyepiece lens. For example, if the objective lens has a magnification of 10x and the eyepiece lens has a magnification of 20x, the total magnification is 10x * 20x = 200x.

It is important to avoid creating air bubbles from the slide when preparing a wet mount because air bubbles can interfere with the clarity and visibility of the specimen. They can also disrupt the refractive index, causing distortion in the image. To avoid air bubbles, you should slowly lower the coverslip onto the specimen at an angle, allowing excess air to escape.