Question

Molecular assays, such as reverse transcription-polymerase chain reaction (RT-PCR), are accurate and sensitive tools for viral infection diagnosis. As a leading CRO company serving both the therapeutic and diagnostic industry, Creative Biolabs always responds quickly to meet the novel requirements of our industrial partners. At this time, we are well-positioned to assist in providing RT-PCR assay development service for detection of SARS-CoV-2, to aid in the reliable diagnosis of suspected cases. sars cov 2 rt pcr

a) Molecular assays
b) RT-PCR
c) Sensitive tools
d) Creative Biolabs

Answer 1

RT-PCR is a molecular assay highly effective for the detection of RNA viruses by converting RNA into cDNA for PCR amplification. It is sensitive and provides quick detection, which is essential for diseases like TB and measuring gene expression. Real-time PCR has enhanced this technique by enabling quantification of viral loads.

Step-by-step explanation:

Reverse transcription-polymerase chain reaction (RT-PCR) is a molecular assay used in virology to detect the presence of RNA viruses, including SARS-CoV-2. Utilizing reverse transcriptase, RT-PCR converts viral RNA into cDNA, which can then be amplified using PCR. This method provides a sensitive tool for viral infection diagnosis and can yield rapid results compared to culture-based methods.

RT-PCR has significant advantages in diagnosing diseases like tuberculosis (TB), as it can detect mycobacteria through tests like the genus-specific 16S rRNA PCR test, species-specific IS6110 PCR test, and others. Moreover, RT-PCR can be used to measure gene expression by converting mRNA into cDNA.

Processes such as real-time PCR (qPCR) have further improved the utility of PCR by allowing quantification of the original target sequence in a sample, which is crucial for determining the extent of viral load.