Question

What are Equipment Required for NGT Insertion Process?

Answer 1

The equipment required for NGT insertion process includes a PCR machine, micropipettes, PCR tubes, and centrifuge tubes. Additional equipment may include disposable test tubes, a mini protean 3 PAGE apparatus, a power supply, UV spectrometer, and a gel documentation system. Other apparatus needed include a flask, measuring cylinder, water bowl, delivery tube, funnel with stopcock, and stopper.

Step-by-step explanation:

1. PCR machine
2. Three sets of micropipettes 20-100 µl, 2-20 μl and 1-10 μl
3. 6 PCR tubes
4. 6 centrifuge tubes

Additional equipment/glassware may include:

1. Six disposable test tubes at least 10 ml volume
2. Three sets of micropipettes 20-100 µl and 2-20 μl
3. Three sets of mini protean 3 PAGE apparatus
4. Power supply (to run urea PAGE)
5. UV spectrometer
6. Gel documentation system
7. Twenty 1.5 ml centrifuge tubes (Eppendorf)
8. Heating block (to boil samples)

Other apparatus needed:

• Flask
• Measuring cylinder
• Water bowl
• Delivery tube
• Funnel with stopcock
• Stopper